Complete nuclear ribosomal DNA sequence analyses of the black-footed abalone Haliotis iris     

Zhansheng Guo  Leng Han 《新西兰海洋与淡水研究杂志》2018,52(2):232-246

The black-footed abalone Haliotis iris is an economically important shellfish species in New Zealand. We successfully amplified, sequenced and analysed the complete nuclear ribosomal DNA (nrDNA) of H. iris. The length of the nrDNA was determined to be around 9.6?kb and included, in order, small subunit ribosomal RNA (nrSSU, 1858bp), internal transcribed spacer (ITS, 749?bp), large subunit ribosomal RNA (nrLSU, 3412bp) and an intergenic spacer (IGS, 3560–3662?bp). The nrLSU genes were identical in two individuals, whereas the nrSSU and ITS regions existed at three and four base differences, respectively. The IGS was more variable than the other nrDNA regions. A phylogenetic tree was constructed based on the ITS sequence datasets, which revealed that Haliotidae has two major subclades, mainly distributed in the North Pacific, Europe and Australia. The complete nrDNA sequence will be useful for the classification, phylogeny and breeding of this shellfish.  相似文献   

基于J2EE架构的企业卡资源管理系统的设计与实现     

任建豪  李成忠 《成都信息工程学院学报》2005,20(5):566-570

介绍了J2EE架构和多层体系结构的发展，在B／S三层架构的基础上给出了包含应用服务器中间件的多层分布式应用体系结构，并把此体系结构应用于电信氽业的有价卡管理系统项目中。分析了此项同的功能结构和体系结构，采用J2EE开发平台和技术设计实现了由浏览器、Web服务器、应用服务器、数据库服务器组成的多层体系架构。  相似文献   

蛤蜊科3种贝类16SrRNA基因片段及ITS2核苷酸序列分析   总被引：4，自引：0，他引：4  

孟学平高如承  董志国阎斌伦程汉良  李艳杰陈建安 《广东海洋大学学报》2006,26(4):8-13

利用PCR技术分别扩增连云港及启东沿海蛤蜊科的西施舌（Coelomactra antiquata）、中国蛤蜊（Mactra chinensis）和四角蛤蜊（Mactra veneriformis）3种双壳贝的16SrRNA基因片段和ITS2核苷酸序列．测序后用DNAstar软件分析了核苷酸差异。结果显示：三种贝类16SrRNA基因片段长度相同，均为306bp（去除引物）．核苷酸存在多态性。共有45个变异位点，54个核苷酸发生了变异。全部为碱基置换。西施舌与中国蛤蜊此片段核苷酸的同源性为88．9％．与四角蛤蜊的同源性为88．6％．中国蛤蜊与四角蛤蜊的同源性为90．6％。三种蛤蜊ITS2序列分别为390bp（西施舌）、441bp（四角蛤蜊）和466bp（中国蛤蜊）。存在长度多态性．ITS2核苷酸差异分析结果显示．西施舌与中国蛤蜊的同源性为70．9％-71．1％，西施舌与四角蛤蜊的为70．5％-71．0％。中国蛤蜊与四角蛤蜊的同源性为88．1％-88．8％。ITS2序列分析结果与16SrRNA基因片段分析结果一致．2种分子分析法均显示中国蛤蜊与四角蛤蜊的亲缘关系近。  相似文献   

REMOTE SENSING FOREST BIOMASS: AN EVALUATION USING HIGH RESOLUTION REMOTE SENSOR DATA AND LOBLOLLY PINE PLOTS     

John R. Jensen  Michael E. Hodgson 《The Professional geographer》1985,37(1):46-56

Eight-year-old Loblolly pine (Pinus taeda) plots located in South Carolina were evaluated using high resolution aircraft multispectral scanner data to determine if biomass could be accurately measured. Sixteen pine plots located on both sandy and clay soils were treated with 0, 180, or 360 kg. (approximately 0, 400, or 800 lb.) of nitrogen per plot. Indices of biomass from remote sensing data were significantly correlated with in situ biomass measurements made in each plot. A ratio of infrared (.9–1.1 μm) and red (.65–.70 μm) channels yielded the best correlation. The indices were not sensitive to differences in soil type (sandy or clay).  相似文献   

数字宜昌建设初探     

魏明果 《地理信息世界》2002,(3)

数字地球、数字城市是近几年刚提出来的一个全新的概念 ,但数字城市的建设却以空前的速度向前发展着 ,国务院总理朱熔基在视察宜昌时提出要把宜昌建设成世界级的城市 ,未来的城市数字化程度将是衡量一个城市发达程度的重要指标 ,启动数字宜昌建设是跻身世界级城市 ,提高宜昌市综合竞争力的重要举措  相似文献   

测绘项目管理实施引论     

龚强 《地理信息世界》2002,(4)

根据我国测绘生产单位的实际情况 ,测绘项目管理的具体实施大致包括对项目的识别、选定、启动与筹集资金、计划、成本估算、执行计划、控制、收尾等主要步骤。当项目确定后 ,需要启动和筹集资金。而计划是项目管理的基本组成部分 ,包括明确项目目标和工作范围。项目成本估算有因素估算法和利用WBS方法的详细估算。项目质量管理规划的依据有质量目标、范围说明、标准和规范等。  相似文献   

Biostratigraphic and aminostratigraphic constraints on the age of the Middle Pleistocene glacial succession in north Norfolk,UK     

Richard C. Preece  Simon A. Parfitt  G. Russell Coope  Kirsty E. H. Penkman  Philippe Ponel  John E. Whittaker 《第四纪科学杂志》2009,24(6):557-580

Considerable debate surrounds the age of the Middle Pleistocene glacial succession in East Anglia following some recent stratigraphical reinterpretations. Resolution of the stratigraphy here is important since it not only concerns the glacial history of the region but also has a bearing on our understanding of the earliest human occupation of north‐western Europe. The orthodox consensus that all the tills were emplaced during the Anglian (Marine Isotope Stage (MIS) 12) has recently been challenged by a view assigning each major till to a different glacial stage, before, during and after MIS 12. Between Trimingham and Sidestrand on the north Norfolk coast, datable organic sediments occur immediately below and above the glacial succession. The oldest glacial deposit (Happisburgh Till) directly overlies the ‘Sidestrand Unio‐bed’, here defined as the Sidestrand Hall Member of the Cromer Forest‐bed Formation. Dating of these sediments therefore has a bearing on the maximum age of the glacial sequence. This paper reviews the palaeobotany and describes the faunal assemblages recovered from the Sidestrand Unio‐bed, which accumulated in a fluvial environment in a fully temperate climate with regional deciduous woodland. There are indications from the ostracods for weakly brackish conditions. Significant differences are apparent between the Sidestrand assemblages and those from West Runton, the type site of the Cromerian Stage. These differences do not result from contrasting facies or taphonomy but reflect warmer palaeotemperatures at Sidestrand and a much younger age. This conclusion is suggested by the higher proportion of thermophiles at Sidestrand and the occurrence of a water vole with unrooted molars (Arvicola) rather than its ancestor Mimomys savini with rooted molars. Amino acid racemisation data also indicate that Sidestrand is significantly younger than West Runton. These data further highlight the stratigraphical complexity of the ‘Cromerian Complex’ and support the conventional view that the Happisburgh Till was emplaced during the Anglian rather than the recently advanced view that it dates from MIS 16. Moreover, new evidence from the Trimingham lake bed (Sidestrand Cliff Formation) above the youngest glacial outwash sediments (Briton's Lane Formation) indicates that they also accumulated during a Middle Pleistocene interglacial – probably MIS 11. All of this evidence is consistent with a short chronology placing the glacial deposits within MIS 12, rather than invoking multiple episodes of glaciation envisaged in the ‘new glacial stratigraphy’ during MIS 16, 12, 10 and 6. Copyright © 2009 John Wiley & Sons, Ltd.  相似文献   

Some thoughts on pulsation and binarity     

Christiaan Sterken 《Astrophysics and Space Science》2006,304(1-4):139-143

Intensive monitoring of main-sequence stars for pulsational studies continuously reveals new binaries and multiple systems. The resulting extensive data sets lead to exact orbital parameters, and to accurate masses of the components. This is also the case higher up the main sequence, though less frequently. We discuss a few examples where intensive observational efforts are necessary in order to improve our knowledge of the pulsational behaviour in the upper main-sequence instability domains, but we emphasize that observational efforts alone that turn out more and more of the same type of data, are by far insufficient, and that there is urgent need for theoretical developments. Determination of highly accurate stellar parameters is of paramount importance and is much more needed than time-series observations.  相似文献   

Source mechanism of mining induced seismic events — Resolution of double couple and non double couple models     

Jan &#x;ílený  Alexander Milev 《Tectonophysics》2008,456(1-2):3

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Peace Parks and jaguar trails: transboundary conservation in a globalizing world   总被引：1，自引：0，他引：1  

Brian King  Sharon Wilcox 《GeoJournal》2008,71(4):221-231

An increasingly utilized strategy for expanding conservation in the developing world has been the promotion of protected areas that supersede national borders. Alternatively known as transfrontier biosphere reserves, transfrontier or transboundary conservation areas, or Peace Parks, these protected areas are aggressively advanced by conservation agencies for their purported ecological and economic benefits. This article provides a comparative assessment of two case studies to understand the various impacts of transboundary conservation. The Great Limpopo Transfrontier Park, which unites protected areas in South Africa, Mozambique and Zimbabwe, is contrasted with efforts to protect jaguars along the United States–Mexico border. We argue that while these cases are promising for the purposes of biodiversity protection, they demonstrate that transboundary conservation can minimize political context, contributes to the hegemony of international conservation agendas, and remains closely linked to economic neoliberalism and decentralization in the developing world.

Brian KingEmail:

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